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1:

The intracellular detection of MIP-1beta enhances the capacity to detect IFN-gamma mediated HIV-1-specific CD8 T-cell responses in a flow cytometric setting providing a sensitive alternative to the ELISPOT.

Kutscher S, Dembek CJ, Allgayer S, Heltai S, Stadlbauer B, Biswas P, Nozza S, Tambussi G, Bogner JR, Stellbrink HJ, Goebel FD, Lusso P, Tinelli M, Poli G, Erfle V, Pohla H, Malnati M, Cosma A.

AIDS Res Ther. 2008 Oct 6;5:22.

PMID: 18837993 [PubMed - in process]

2:

Expression of the chemokine MIG is a sensitive and predictive marker for antigen-specific, genetically restricted IFN-gamma production and IFN-gamma-secreting cells.

Brice GT, Graber NL, Hoffman SL, Doolan DL.

J Immunol Methods. 2001 Nov 1;257(1-2):55-69.

PMID: 11687239 [PubMed - indexed for MEDLINE]

3:

Frequencies of ex vivo-activated human immunodeficiency virus type 1-specific gamma-interferon-producing CD8+ T cells in infected children correlate positively with plasma viral load.

Buseyne F, Scott-Algara D, Porrot F, Corre B, Bellal N, Burgard M, Rouzioux C, Blanche S, Rivière Y.

J Virol. 2002 Dec;76(24):12414-22.

PMID: 12438567 [PubMed - indexed for MEDLINE]

5:

A dual color ELISPOT method for the simultaneous detection of IL-2 and IFN-gamma HIV-specific immune responses.

Boulet S, Ndongala ML, Peretz Y, Boisvert MP, Boulassel MR, Tremblay C, Routy JP, Sekaly RP, Bernard NF.

J Immunol Methods. 2007 Mar 30;320(1-2):18-29. Epub 2006 Dec 29.

PMID: 17222422 [PubMed - indexed for MEDLINE]

6:

Optimization and validation of an 8-color intracellular cytokine staining (ICS) assay to quantify antigen-specific T cells induced by vaccination.

Horton H, Thomas EP, Stucky JA, Frank I, Moodie Z, Huang Y, Chiu YL, McElrath MJ, De Rosa SC.

J Immunol Methods. 2007 May 31;323(1):39-54. Epub 2007 Apr 3.

PMID: 17451739 [PubMed - indexed for MEDLINE]

7:

Intracellular staining for HIV-specific IFN-gamma production: statistical analyses establish reproducibility and criteria for distinguishing positive responses.

Trigona WL, Clair JH, Persaud N, Punt K, Bachinsky M, Sadasivan-Nair U, Dubey S, Tussey L, Fu TM, Shiver J.

J Interferon Cytokine Res. 2003 Jul;23(7):369-77.

PMID: 14511463 [PubMed - indexed for MEDLINE]

8:

[Evolution of IGRA researches]

Ariga H, Harada N.

Kekkaku. 2008 Sep;83(9):641-52. Review. Japanese.

PMID: 18979999 [PubMed - indexed for MEDLINE]

9:

Phenotypic and functional analysis of immune CD8+ T cell responses induced by a single injection of a HIV DNA vaccine in mice.

Arrode G, Hegde R, Mani A, Jin Y, Chebloune Y, Narayan O.

J Immunol. 2007 Feb 15;178(4):2318-27.

PMID: 17277137 [PubMed - indexed for MEDLINE]

10:

A systematic comparison of methods to measure HIV-1 specific CD8 T cells.

Sun Y, Iglesias E, Samri A, Kamkamidze G, Decoville T, Carcelain G, Autran B.

J Immunol Methods. 2003 Jan 15;272(1-2):23-34.

PMID: 12505709 [PubMed - indexed for MEDLINE]

11:

HIV-1 MN Env 15-mer peptides better detect HIV-1 specific CD8 T cell responses compared with consensus subtypes B and M group 15-mer peptides.

Rutebemberwa A, Currier JR, Jagodzinski L, McCutchan F, Birx D, Marovich M, Cox JH.

AIDS. 2005 Jul 22;19(11):1165-72.

PMID: 15990569 [PubMed - indexed for MEDLINE]

12:

A comparison of standard immunogenicity assays for monitoring HIV type 1 gag-specific T cell responses in Ad5 HIV Type 1 gag vaccinated human subjects.

Tobery TW, Dubey SA, Anderson K, Freed DC, Cox KS, Lin J, Prokop MT, Sykes KJ, Mogg R, Mehrotra DV, Fu TM, Casimiro DR, Shiver JW.

AIDS Res Hum Retroviruses. 2006 Nov;22(11):1081-90.

PMID: 17147493 [PubMed - indexed for MEDLINE]

13:

Synergistic effect of combined HIV/HCV immunogens: a combined HIV-1/HCV candidate vaccine induces a higher level of CD8+ T cell-immune responses in HLA-A2.1 mice.

Azizi A, Ghorbani M, Soare C, Mojibian M, Diaz-Mitoma F.

Curr HIV Res. 2007 Mar;5(2):211-9.

PMID: 17346135 [PubMed - indexed for MEDLINE]

15:

Tuberculosis antigen-specific immune responses can be detected using enzyme-linked immunospot technology in human immunodeficiency virus (HIV)-1 patients with advanced disease.

Clark SA, Martin SL, Pozniak A, Steel A, Ward B, Dunning J, Henderson DC, Nelson M, Gazzard B, Kelleher P.

Clin Exp Immunol. 2007 Nov;150(2):238-44. Epub 2007 Aug 2.

PMID: 17672869 [PubMed - indexed for MEDLINE]

16:

Measuring T-cell immunity in HIV vaccine clinical trials: PBMC and ELISPOT assay proficiency concordance in laboratories from 3 continents.

Boaz MJ, Hayes P, Tarragona T, Seamons L, Cooper A, Birungi J, Kitandwe P, Semaganda A, Kaleebu P, Stevens G, Anzala O, Farah B, Ogola S, Indangasi J, Mhlanga P, Van Eeden M, Thakar M, Pujari A, Mishra S, Goonetilleke N, Moore S, Mahmoud A, Sathyamoorthy P, Mahalingam J, Narayanan PR, Ramanathan VD, Cox JH, Dally L, Gill DK, Gilmour J.

Clin Vaccine Immunol. 2008 Dec 17. [Epub ahead of print]

PMID: 19091991 [PubMed - as supplied by publisher]

17:

Comparison of the ELISPOT and cytokine flow cytometry assays for the enumeration of antigen-specific T cells.

Karlsson AC, Martin JN, Younger SR, Bredt BM, Epling L, Ronquillo R, Varma A, Deeks SG, McCune JM, Nixon DF, Sinclair E.

J Immunol Methods. 2003 Dec;283(1-2):141-53.

PMID: 14659906 [PubMed - indexed for MEDLINE]

18:

The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity.

Shafer-Weaver K, Sayers T, Strobl S, Derby E, Ulderich T, Baseler M, Malyguine A.

J Transl Med. 2003 Dec 29;1(1):14.

PMID: 14697097 [PubMed - as supplied by publisher]

19:

Detection of antigen-specific T cell interferon gamma expression by ELISPOT and cytokine flow cytometry assays in rhesus macaques.

Pahar B, Li J, Rourke T, Miller CJ, McChesney MB.

J Immunol Methods. 2003 Nov;282(1-2):103-15.

PMID: 14604545 [PubMed - indexed for MEDLINE]

20:

Substantial intrapatient differences in the breadth and specificity of HIV-specific CD8+ T-cell interferon-gamma and proliferation responses.

McKinnon LR, Ball TB, Wachihi C, Chinga N, Maingi A, Luo M, Fowke KR, Plummer FA.

J Acquir Immune Defic Syndr. 2008 Oct 1;49(2):123-7.

PMID: 18769359 [PubMed - indexed for MEDLINE]

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