Table 1

Screening of Various CDK and NFkB/IKK Inhibitors and Related Molecules for HTLV-1 Cell Killing
Selectivity
 Name
 MT-2
 MT-4
 C8166
 C10/MJ
 CEM
 Jurkat
 Reported activities of molecules (IC50 in nM)



Infected
 Uninfected
High
 BMS-345541
 (10 uM)
 ++++*
 +++
 +++
 +
 - **
 -
 IKK-1(4), IKK-2(0.3)

Purvalanol A
 (10 uM)
 ++++
 ++
 ++
 -
 -
 -
 CDK1(4), CDK2(70), CDK5(75)

Indirubin-3'-monoxime
 (10 uM)
 +++
 +++
 +++
 +++
 ++
 ++
 CDK1(180), CDK2(250), CDK4(3330), CDK5(100)

Indirubin-3'-monoxime-5'-Iodo
 (10 uM)
 +++
 +++
 +++
 +++
 ++
 ++
 CDK1(25), CDK5(20)

9-Cyanopaullone
 (10 uM)
 ++++
 +
 -
 -
 -
 -
 CDK1(24), CDK5(44)

Aloisine A
 (10 uM)
 +++
 +
 -
 -
 -
 -
 CDK1(150), CDK2(120), CDK5(200)

Compound 52
 (10 uM)
 +++
 +
 -
 -
 -
 -
 CDK1(340)

Flavopiridol
 (0.1 uM)
 ++
 +
 -
 -
 -
 -
 CDK9(50)
Moderate
 r-Roscovitine
 (10 uM)
 ++
 -
 -
 -
 -
 -
 CDK1(650), CDK2(700), CDK5(160), CDK7(500)

Bohemine
 (10 uM)
 ++
 -
 -
 -
 -
 -
 CDK1(1000)

s-Roscovitine
 (10 uM)
 +
 -
 -
 -
 -
 -
 CDK1(650), CDK2(700), CDK5(160), CDK7(500)

WHI-P180
 (10 uM)
 +
 N/A
 N/A
 -
 N/A
 N/A
 CDK2(1000)

Kenpaullone
 (10 uM)
 +
 -
 -
 -
 -
 -
 CDK1(400), CDK2(680), CDK5(850)

2,6-Diaminopurine
 (10 uM)
 -
 +
 -
 -
 -
 -

Flavone
 (10 uM)
 +
 -
 -
 -
 -
 -
 CDK1(300), CDK2(100), CDK4(400), CDK7(300)

Alsterpaullone
 (10 uM)
 ++++
 ++
 +++
 +
 ++
 ++
 CDK1(35), CDK2(15), CDK5(40)

CGP 74514A
 (10 uM)
 ++++
 ++
 +++
 +
 ++
 ++
 CDK1(25)

BAY 11-7085
 (10 uM)
 +
 ++++
 +++
 N/A
 ++++
 ++++

BAY 11-7082
 (10 uM)
 +
 ++++
 +++
 N/A
 ++++
 ++++
 IkBa(10000)

CAPE
 (10 uM)
 +
 +++
 -
 N/A
 ++++
 ++++

Diethylmaleate
 (10 uM)
 +
 +++
 -
 N/A
 ++++
 ++++

Parthenolide
 (10 uM)
 +
 +++
 +++
 N/A
 ++++
 ++++

Pyrrolidinedithiocarbamic acid
 (10 uM)
 -
 +++
 +++
 N/A
 ++++
 ++++

Wedelolactone
 (10 uM)
 +
 ++
 -
 N/A
 ++
 ++
Poor
 6-Benzyloxypurine
 (10 uM)
 -
 -
 -
 -
 -
 -

5-amino alicylic acid
 (10 uM)
 -
 -
 -
 N/A
 -
 -

2,6-Dichloropurine
 (10 uM)
 -
 -
 -
 -
 -
 -

6-Dimethylaminopurine
 (10 uM)
 -
 -
 -
 -
 -
 -
 CDC

Indirubin-3'-monoxime-5'-sulphonic acid
 (10 uM)
 -
 -
 -
 -
 -
 -
 CDK1(5), CDK5(7)

Iso-olomoucine
 (10 uM)
 -
 -
 -
 -
 -
 -
 CDK1(>500,000), CDK4,5(>1,000,000)

N-6-(Δ2-Isopentyl)-adenine
 (10 uM)
 -
 -
 -
 -
 -
 -
 CDK1,2,5(>50,000)

Olomoucine
 (10 uM)
 -
 -
 -
 -
 -
 -
 CDK1,2(7000), CDK5(3000)

Olomoucine, N9-isopropyl
 (10 uM)
 -
 -
 -
 -
 -
 -
 CDK1(2000)

SC-514
 (10 uM)
 -
 -
 -
 -
 -
 -
 IKK-1(>200), IKK-2(11.2)

QNZ
 (10 uM)
 -
 -
 -
 N/A
 ++
 +

* positive sign indicates level of cell inhibition
Inhibition
 Percentage





** negative sign indicates no cell inhibition
-
 1–5%







+
 25%







++
 50%







+++
 75%







++++
 90%




Agbottah et al. AIDS Research and Therapy 2008 5:12   doi:10.1186/1742-6405-5-12