Additional file 4.

Flow cytometry profiles of Jurkat T-cells transduced with HIV-1 vectors encoding EGFP or EGFP-2A-Rev M10. The packaging constructs used for preparation of the vector stocks are shown on the Y-axis while the gene transfer vectors are shown on the X-axis. The attributes of the gene transfer vectors, such as the presence of HIV-1 or SIV RRE, the transgene expressed (EGFP or EGFP-2A-M10) are indicated. GFP expression is depicted along the X-axis and forward scatter (FSC) is indicated along the Y-axis. The percentage and the geometric mean of fluorescence intensity (GMFI) of the EGFP positive populations are shown. Vector stocks for transductions shown in panels A through H were produced using pCI-HIV-Rev while those for transductions in J and K used pCI-SIV-Rev. Representative data from two independent experiments.

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Srinivasakumar AIDS Research and Therapy 2008 5:11   doi:10.1186/1742-6405-5-11