Figure 2.

A) Representative section of the brain biopsy (H&E 40X) shows thickened leptomeninges with an infiltrate composed of lymphocytes and plasmocytes that extended perivascularly. Inset shows an astrocyte with prominent and hyperchromatic nuclei. B) Electrophoresis on 2% agarose gel of BKV PCR products from CSF. Lane 1, Marker 100 bp: Lane 2, negative control of PCR; Lane 3, negative control of extraction; Lane 4, 250 ng DNA; Lane 5, positive control of PCR. C) Electrophoresis on 2% agarose gel of JCV PCR products from brain biopsy. Lane 1, Marker 100 bp; Lane 2, negative control of PCR; Lane 3, negative control of extraction; Lane 4,1000 ng DNA; Lane 5, positive control of PCR.