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HIV post exposure prophylaxis induced bicytopenia: a case report

Benjamin T Schleenvoigt13*, John P Fobiwe1, Peter M Keller13, Stefan Hagel124 and Mathias W Pletz14

Author Affiliations

1 Center for Infectious Diseases and Infection Control, Jena University Hospital, Erlanger Allee 101, Jena, Thuringia 07740, Germany

2 Department of Internal Medicine IV, Jena University Hospital, Jena, Germany

3 Institute for Medical Microbiology, Jena University Hospital, Jena, Germany

4 Center for Sepsis Control and Care (CSCC), Jena University Hospital, Jena, Germany

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AIDS Research and Therapy 2014, 11:11  doi:10.1186/1742-6405-11-11

The electronic version of this article is the complete one and can be found online at:

Received:5 November 2013
Accepted:1 February 2014
Published:7 February 2014

© 2014 Schleenvoigt et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.


Long and short term side effects of antiretroviral drugs are not fully understood yet. Here a case of reversible blood count changes following post exposure prophylaxis with tenofovir/emtricitabin and lopinavir/ritonavir is reported. We propose that antiretroviral drugs used in post exposure prophylaxis may have a significant impact on hematopoiesis.

HIV; Post exposure prophylaxis; Leukopenia; Thrombocytopenia; Tenofovir; Emtricitabin; Lopinavir; Ritonavir


Needle prick injury is common in clinical practice. In order to prevent HIV infection, post exposure prophylaxis (PEP) is considered in situations with potential risk of infection [1,2]. Long and short term side effects of the drugs used are not fully understood yet. Here, we report a case of reversible leukopenia and thrombocytopenia following a 28 days course of post exposure prophylaxis with tenofovir/emtricitabin and lopinavir/ritonavir.

Case presentation

A 56 years old male patient presented after occupational needle prick injury. Index patient could not be determined and PEP was started within 28 hours with lopinavir 400 mg/ritonavir 100 mg BD and tenofovir 245 mg/emtricitabin 200 mg QD and was continued for 28 days. Serology for HIV, HCV, HBV as well as parameters for blood count (leukocytes 4.6 Gpt/l, thrombocytes 146 Gpt/l), liver and renal function tests were unremarkable. Hepatitis B vaccination was administered. Past medical history revealed coronary heart disease, hypertension and the patient reported known marginal reduction of platelets in absence of any hemic disease. The concurrent medication consisted of ramipril 5 mg QD, acetylsalicylacid 100 mg QD and simvastatin 40 mg QD. The statin was paused during PEP.

Antiretroviral post exposure treatment was clinically well tolerated and the patient reported no symptoms of rash or gastrointestinal side effects. Control of laboratory parameters on day 19 after initiation of PEP showed a slight decrease in WBC to 4.0 Gpt/l. Investigation on day 33 (5 days after the end of PEP) showed bicytopenia with leukopenia 2.0 Gpt/l and thrombocytopenia 97 Gpt/l. A second control on day 40 revealed a return to a normal blood count and no alterations of differential blood count (neutrophil granulocytes 3.61 Gpt/l, lymphocytes 1.46 Gpt/l, monocytes 0.39 Gpt/l, eosinophil granulocytes 0.06 Gpt/l, basophil granulocytes 0.02 Gpt/l). Serum electrophoresis was unremarkable (total protein 70.4 g/l, albumin 66.9%, alpha-1-globulin 3.6%, alpha-2-globulin 8.4%, beta-globulin 9.7%, gamma-globulin 11.4%) and determination of ANA and pANCA as well as folic acid and vitamin B 12 levels revealed normal values. There was no evidence of blood count changes during follow up over 6 months and the patient remained sero-negative for HIV and HCV. After stratification of benefits and risks, no further invasive clarification of pathogenicity was initiated.


Cytopenia such as anemia, thrombocytopenia, neutropenia or lymphopenia is a known effect of HIV and AIDS status is an identified risk factor. A recent Korean publication pointed out the impact of HIV alone on hematologic manifestations. In this study cytopenia was shown to be reversible with antiretroviral treatment [3]. Thrombocytopenia or leukopenia following antiretroviral post exposure therapy with tenofovir or emtricitabin have not been described yet. In a retrospective study leukopenia was associated with lopinavir/ritonavir [4]. A thorough review revealed a single case of thrombocytopenia associated with lopinavir/ritonavir [5]. As a mechanism of pathogenicity autoimmune causes can be discussed for thrombocytopenia in our case. Since ANA and ANCA were tested negative this hypothesis is less convincible. As leukopenia emerged simultaneously to thrombocytopenia a direct impact on hematopoiesis seems more plausible. Thrombocytopenia was described for other protease inhibitors such as saquinavir, but to this date no feasible hypothesis for pathogenicity is available [6]. Based on previous observations and this case report we propose that antiretroviral drugs used in PEP may have a direct impact on hematopoiesis. The precise mechanism should be further investigated.


Written informed consent was obtained from the patient for publication of this case report. A copy of the written consent is available for review by the Editor-in-Chief of this journal.


AIDS: Acquired immunodeficiency syndrome; ANA: Anti-nuclear antibody; ANCA: Anti-neutrophil cytoplasmic antibody; HBV: Hepatitis B virus; HCV: Hepatitis C virus; HIV: Human immunodeficiency virus; PEP: Post exposure prophylaxis; WBC: White blood cells.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

The patient was treated and followed up by BTS and JPF. BTS conceived the case report, did the literature research and drafted the manuscript. It was critically corrected by PMK and SH. MWP helped to draft the manuscript and participated in the literature research and coordination. All authors read and approved the final manuscript.


This work was supported by Bundesministerium für Bildung und Forschung (BMBF); grant to Mathias W. Pletz (01 KI 1204).


  1. Gemeinsame Erklarung der Deutschen A-G: Post-exposure prophylaxis of HIV infection. German-Austrian recommendations, update September 2007.

    Dtsch Med Wochenschr 2009, 134(Suppl 1):S16-33. PubMed Abstract | Publisher Full Text OpenURL

  2. Guidelines Version 7.0 October 2013 webcite

  3. Choi SY, Kim I, Kim NJ, Lee SA, Choi YA, Bae JY, Kwon JH, Choe PG, Park WB, Yoon SS, et al.: Hematological manifestations of human immunodeficiency virus infection and the effect of highly active anti-retroviral therapy on cytopenia.

    Korean J Hematol 2011, 46:253-257. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  4. Alp E, Bozkurt I, Doganay M: Epidemiological and clinical characteristics of HIV/AIDS patients followed-up in Cappadocia region: 18 years experience.

    Mikrobiyol Bul 2011, 45:125-136. PubMed Abstract OpenURL

  5. Colebunders R, De Schacht C, Vanwolleghem T, Callens S: Lopinavir/ritonavir- and indinavir-induced thrombocytopenia in a patient with HIV infection.

    Int J Infect Dis 2004, 8:315-316. PubMed Abstract | Publisher Full Text OpenURL

  6. Fellay J, Boubaker K, Ledergerber B, Bernasconi E, Furrer H, Battegay M, Hirschel B, Vernazza P, Francioli P, Greub G, et al.: Prevalence of adverse events associated with potent antiretroviral treatment: swiss HIV cohort study.

    Lancet 2001, 358:1322-1327. PubMed Abstract | Publisher Full Text OpenURL