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Open Access Short report

Differential inflammasome expression and IL-1β secretion in monocyte-derived dendritic cells differentiated with IL-4 or IFN-α

Alessandra Pontillo1*, Bruna T Santillo2, Alberto JS Duarte2 and Telma M Oshiro2

Author Affiliations

1 Laboratory of Immunogenetics, Departament of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil

2 Laboratory of Medical Investigation in Dermatology and Immunodeficiency, LIM-56, Faculty of Medicine, University of Sao Paulo, Sao Paulo, Brazil

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AIDS Research and Therapy 2013, 10:35  doi:10.1186/1742-6405-10-35

Published: 27 December 2013

Abstract

Background

NLRP3-inflammasome activation was evaluated in monocyte-derived dendritic cells (DC) obtained through IL-4 (IL4-DC) or IFN-α (IFN-DC) protocols and pulsed with chemically inactivated HIV-1. Inflammasome’ genes expression and IL-1β secretion were compared in DC isolated from 15 healthy subjects (HC) and 10 HIV-1 infected individuals (HIV+).

Findings

Whether HIV was able to increased NLRP3-inflammasome genes expression and IL-1β secretion in IL4-DC from HC, the induction of inflammasome appeared significantly reduced in IFN-DC from HC, suggesting a different responsive state of IFN-DC compared to IL4-DC. No inflammasome activation was observed in IL4-DC as well as in IFN-DC derived from HIV + subjects, confirming previous findings on “unresponsive” state of DC derived from HIV + possibly due to chronic inflammatory state of these individuals.

Conclusions

Our results showed that IFN-α differently modulates inflammasome expression during monocytes-DC in vitro differentiation. These findings could be of interest considering the on-going research about DC manipulation and therapeutic strategies for HIV + involving DC-based immune-vaccines.

Keywords:
HIV-1; Type-1 IFN; Dendritic cells; Inflammasome; NALP3; IL-1β; Vaccine; Immunotherapy